Enem Patogen Pernafasan
jeneng produk
Kit Deteksi Asam Nukleat HWTS-RT175-Enam Patogen Pernafasan (PCR Fluoresensi)
Epidemiologi
Infeksi ambegan minangka klompok penyakit manungsa sing paling umum sing bisa kedadeyan ing jender, umur lan wilayah geografis lan minangka salah sawijining panyebab morbiditas lan kematian sing paling penting ing populasi ing saindenging jagad. Patogen pernapasan klinis umum kalebu virus respiratory syncytial, adenovirus, metapneumovirus manungsa, rhinovirus, virus parainfluenza (I/II/III) lan Mycoplasma pneumoniae. Tandha lan gejala klinis sing disebabake infeksi saluran pernapasan relatif padha, nanging perawatan, khasiat, lan durasi penyakit beda-beda ing antarane infeksi sing disebabake dening patogen sing beda. Saiki, cara utama kanggo deteksi laboratorium saka patogen pernapasan ing ndhuwur kalebu: isolasi virus, deteksi antigen lan deteksi asam nukleat. Kit iki mbantu diagnosa infeksi virus pernapasan kanthi ndeteksi lan ngenali asam nukleat virus tartamtu ing individu sing duwe tandha lan gejala infeksi pernapasan, kanthi kombinasi temuan klinis lan laboratorium liyane.
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | Sampel swab orofaringeal |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | LoD saka Adv, MP, RSV, hMPV, RhV lan PIV kabeh 200 Salinan/mL |
| Kekhususan | Asil tes reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit lan novel coronavirus, virus influenza A, virus influenza B, human bocavirus, cytomegalovirus, virus herpes simplex tipe 1, virus varicella zoster, EBV, bacillus pertussis, Chlamydophila pneumoniae, Corynebacterium colitus, Ezacilillus spp spp, Legionella pneumophila, C. catarrhalis, lan galur Mycobacterium tuberculosis sing dilemahkan, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyococcus, Streptococcus pyococcus baumannii, Monokokus maltofilik pakan sempit, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspergillus fumigatus, Aspergillus flavus, Pneumatobacteria spp, Candida alkitab, Rohycerabicnogal streptococci, Klebsiella pneumoniae, Chlamydia psittaci, demam Rickettsia q lan asam nukleat genom manungsa. Kemampuan anti-interfering: mucin (60 mg/mL), getih manungsa, benfotiamine (2 mg/mL), oxymetazoline (2 mg/mL), natrium klorida (20 mg/mL), beclomethasone (20 mg/mL), deksametason (20 mg/mL), flunitrazolone (20 μg/mL/mL), fludesnitrazolone (20 μg/mL/1), triamcinide mg/mL), mometasone (2 mg/mL), flutikason (2 mg/mL), histamin hidroklorida (5 mg/mL), vaksin virus influenza langsung intranasal, benzocaine (10%), mentol (10%), zanamivir (20 mg/mL), ribavirin (10 mg/L), paramivir (10 mg/L), paramivir (10 mg/L), paramivir (1 mg/mL), (20 mg/mL), tobramycin (0,6 mg/mL), UTM, saline, guanidine hydrochloride (5 M/L), Tris (2 M/L), ENTA-2Na (0,6 M/L), trilostane (15%), isopropil alkohol (20%), lan kalium klorida (1 M/L) sing dites asil ora ana interferensi reaksi. patogen ing konsentrasi ing ndhuwur zat interfering. |
| Instrumen sing ditrapake | Sistem PCR Real-Time SLAN-96P Applied Biosystems 7500 Real-Time PCR System QuantStudio®5 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Kit DNA/RNA Umum Makro & Uji Mikro (HWTS-3019) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Makro & Mikro (HWTS-3006C, (HWTS-3006B))dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. dianjurake kanggo ekstraksi sampel lanlangkah sakteruse kudukondukted ing selaras ketat karo IFUsaka Kit.
