Patogen Pernafasan Gabungan
jeneng produk
Kit Deteksi Gabungan Patogen Pernapasan HWTS-RT183 (PCR Fluoresensi)
Epidemiologi
Penyakit Virus Corona 2019, diarani 'COVID-19', nuduhake pneumonia sing disebabake dening infeksi SARS-CoV-2. SARS-CoV-2 minangka coronavirus sing kalebu genus β. COVID-19 minangka penyakit infèksi pernapasan akut, lan populasi umume rentan. Saiki, sumber infeksi utamane yaiku pasien sing kena infeksi 2019-nCoV, lan wong sing kena infeksi asimtomatik uga bisa dadi sumber infeksi. Adhedhasar penyelidikan epidemiologis saiki, periode inkubasi yaiku 1-14 dina, biasane 3-7 dina. Demam, batuk garing lan lemes minangka manifestasi utama. Sawetara pasien nandhang gejala kayata hidung tersumbat, irung meler, tenggorokan perih, myalgia lan diare, lan liya-liyane. Influenza, sing umum dikenal minangka 'flu', yaiku penyakit infeksi saluran pernapasan akut sing disebabake dening virus influenza. Iku banget infèksius. Biasane ditularake kanthi watuk lan wahing. Biasane pecah ing musim semi lan musim dingin. Virus influenza dipérang dadi influenza A (IFV A), influenza B (IFV B), lan Influenza C (IFV C) telung jinis, kabeh kalebu virus lengket, nyebabake penyakit manungsa utamane kanggo virus influenza A lan B, yaiku virus RNA untai tunggal, segmen. Virus influenza A minangka infeksi saluran pernapasan akut, kalebu H1N1, H3N2 lan subtipe liyane, sing rawan mutasi lan wabah ing saindenging jagad. 'Shift' nuduhake mutasi virus influenza A, sing nyebabake muncule 'subtipe' virus anyar. Virus influenza B dipérang dadi rong garis keturunan, yamagata lan virus Influenza Victoria B mung duwé panyimpangan antigen, lan ora bisa ngawasi lan ngilangi sistem kekebalan manungsa liwat mutasi. Nanging, kacepetan évolusi virus influenza B luwih alon tinimbang virus influenza A manungsa. Virus influenza B uga bisa nyebabake infeksi pernapasan manungsa lan nyebabake epidemi.
Respiratory syncytial virus (RSV) yaiku virus RNA, kalebu kulawarga paramyxoviridae. Iki ditularake kanthi tetesan udara lan kontak sing cedhak lan minangka patogen utama infeksi saluran napas ngisor ing bayi. Bayi sing kena infeksi RSV bisa ngalami bronchiolitis lan radhang paru-paru sing abot, sing ana hubungane karo asma ing bocah-bocah. Bayi duwe gejala sing abot, kalebu demam dhuwur, rhinitis, pharyngitis lan laringitis, banjur bronchiolitis lan radhang paru-paru. Sawetara bocah sing lara bisa dadi rumit karo otitis media, pleurisy lan miokarditis, lan liya-liyane. Infeksi saluran napas ndhuwur minangka gejala utama infeksi ing wong diwasa lan bocah sing luwih tuwa.
Parameter teknis
| Panyimpenan | -18 ℃ Ing peteng |
| urip beting | 9 wulan |
| Tipe Spesimen | swab oropharyngeal; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, SARS-CoV-2, IFV A H1N1Ct≤38 |
| CV | ≤5% |
| LoD | 200 eksemplar/μL |
| Kekhususan | Hasil cross-reactivity nuduhake yen ora ana reaksi silang antarane kit lan cytomegalovirus, virus herpes simplex tipe 1, virus varicella zoster, virus Epstein-Barr, adenovirus, metapneumovirus manungsa, rhinovirus, virus parainfluenza tipe I/II/III/IV, bocavirus, enterovirus, coronavirus, Mycoplasmadia pneumoniaesis, Chlamydia pneumoniae, Chlamydia pneumoniae, spp., Escherichia coli, Haemophilus influenzae, Lactobacillus spp., Legionella pneumophila, Moraxella catarrhalis, galur Mycobacterium tuberculosis sing dilemahkan, Neisseria meningitidis, Neisseria spp., Pseudomonas aeruginosaure pneumonia, Staphylococcus a. epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium fasciatum, Nocardia, Serratia marcescens, Citrobacter rodentium, Cryptococcus, Aspergillus Aspergimigallus carinii, Candida albicans, Roseburia mucosa, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia Q mriyang lan asam nukleat genomik manungsa. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time, Applied Biosystems 7500 Sistem PCR Real-Time Cepet, QuantStudio®5 Sistem PCR Wektu Nyata, Sistem PCR Wektu Nyata SLAN-96P (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Sistem PCR Real-Time, LineGene 9600 Plus Sistem Deteksi PCR Real-Time (FQD-96A, teknologi Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX.Opus 96 Real-Time PCR System |
Alur Kerja
Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Nukleat Otomatis Makro & Mikro (HWTS-3006C, HWTS-3006B)), lan Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-8 bisa digunakake)TM AIO800 (HWTS-EQ007)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Volume sampel sing diekstrak yaiku 200μL lan volume elusi sing disaranake yaiku 150μL.
