17 Jinis HPV (16/18/6/11/44 Ngetik)
jeneng produk
HWTS-CC015 17 Jinis Human Papillomavirus (Pengetikan 16/18/6/11/44) Kit Deteksi Asam Nukleat (PCR Fluoresensi)
Epidemiologi
Kanker serviks minangka salah sawijining tumor ganas sing paling umum ing saluran reproduksi wanita.Wis ditampilake yen infeksi HPV sing terus-terusan lan macem-macem infeksi minangka salah sawijining panyebab utama kanker serviks.Saiki isih kurang perawatan efektif kanggo kanker serviks sing disebabake dening HPV.Mula, deteksi dini lan nyegah infeksi serviks sing disebabake dening HPV minangka kunci kanggo nyegah kanker serviks.Nggawe tes diagnostik sing prasaja, spesifik lan cepet kanggo patogen penting banget kanggo diagnosa klinis kanker serviks.
Saluran
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Kontrol internal |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Kontrol internal |
Parameter teknis
| Lumbung | -18 ℃ |
| urip beting | 12 sasi |
| Tipe Spesimen | Sampel urin, sampel swab serviks wanita, sampel swab vagina wanita |
| Ct | ≤28 |
| LoD | 300 eksemplar / ml |
| Kekhususan | Ora ana reaksi silang karo Ureaplasma urealyticum, Chlamydia trachomatis saka saluran reproduksi, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mould, Gardnerella lan jinis HPV liyane sing ora kalebu kit. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 200μL saline normal kanggo resuspensi pelet ing langkah 2.1, banjur ekstraksi kudu ditindakake miturut kanggo pandhuan kanggo nggunakake reagen ekstraksi iki.Volume elusi sing disaranake yaiku 80μL.
Reagen ekstraksi sing disaranake: QIAamp DNA Mini Kit (51304) utawa Kolom DNA/RNA Viral Macro & Micro-Test (HWTS-3020-50).Tambah 200μL saline normal kanggo resuspend pelet ing langkah 2.1, lan banjur extraction kudu conducted miturut pandhuan kanggo nggunakake reagen extraction iki.Volume sampel sing diekstrak kabeh 200μL, lan volume elusi sing disaranake yaiku 100μL.
Reagen ekstraksi sing disaranake: Reagen Pelepasan Sampel Macro & Micro-Test (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Tambah 200μL saka sampel release reagen kanggo resuspend pelet ing langkah 2.1, lan banjur extraction kudu conducted miturut pandhuan kanggo nggunakake reagen extraction iki.
