Virus Polio Ⅰ
jeneng produk
HWTS-EV006- Tipe Virus Polio Ⅰ Kit Deteksi Asam Nukleat (PCR Fluoresensi)
Epidemiologi
Poliovirus yaiku virus sing nyebabake poliomielitis, penyakit infèksi akut sing nyebar sacara wiyar.Virus iki kerep nyerang sistem saraf pusat, ngrusak sel saraf motor ing sungu anterior sumsum tulang belakang, lan nyebabake kelumpuhan flaksid saka anggota awak, sing luwih umum ing bocah-bocah, mula uga diarani polio.Poliovirus kalebu genus enterovirus saka kulawarga picornaviridae.Poliovirus nyerang awak manungsa lan nyebar utamane liwat saluran pencernaan.Bisa dipérang dadi telung serotipe miturut kekebalan, jinis I, jinis II, lan jinis III.
Saluran
| FAM | virus polio tipe I |
| ROX | Kontrol internal |
Parameter teknis
| Lumbung | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | Sampel bangku sing anyar diklumpukake |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 1000 eksemplar / ml |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®5 Sistem PCR Real-TimeSistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ekstraksi kudu ditindakake miturut IFU kanthi ketat. Volume elusi sing disaranake yaiku 80μL.
Pilihan 2.
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3022) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ekstraksi kudu ditindakake miturut IFU kanthi ketat.Volume elusi sing disaranake yaiku 100μL.
