Mycobacterium Tuberculosis Nucleic Acid and Rifampicin (RIF), Isoniazid Resistance (INH)

Katrangan singkat:

Produk iki cocok kanggo deteksi kualitatif DNA Mycobacterium tuberculosis ing sampel sputum manungsa ing vitro, uga mutasi homozigot ing wilayah kodon asam amino 507-533 (81bp, wilayah penentu resistensi rifampisin) saka gen rpoB sing nyebabake Mycobacterium tuberculosis resistensi rifampisin.

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Detail Produk

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jeneng produk

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)

Epidemiologi

Mycobacterium tuberculosis, sakcepete minangka Tubercle bacillus (TB), yaiku bakteri patogen sing nyebabake tuberkulosis.Saiki, obat anti-tuberkulosis lini pertama sing umum digunakake kalebu isoniazid, rifampicin lan etambutol, lan liya-liyane. Obat anti-tuberkulosis baris kapindho kalebu fluoroquinolones, amikacin lan kanamycin, lan liya-liyane. Obat-obatan anyar sing dikembangake yaiku linezolid, bedaquiline lan delamani, lsp. Nanging, amarga nggunakake obat anti-tuberkulosis sing ora bener lan karakteristik struktur tembok sel mycobacterium tuberculosis, mycobacterium tuberculosis ngembangake resistensi obat kanggo obat anti-tuberkulosis, sing ndadekake tantangan serius kanggo nyegah lan ngobati tuberkulosis.

Saluran

Jeneng Target			Wartawan	Quencher
Buffer ReaksiA	Buffer ReaksiB	Buffer ReaksiC	Wartawan	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	ora ana
rpoB 520-527	rpoB 527-533	/	CY5	ora ana
/	/	Kontrol internal	HEX(VIC)	ora ana

Buffer ReaksiD	Wartawan	Quencher
Wilayah promotor InhA -15C>T, -8T>A, -8T>C	FAM	ora ana
KatG 315 kodon 315G>A、315G>C	CY5	ora ana
wilayah promotor AhpC -12C>T, -6G>A	ROX	ora ana

Parameter teknis

Lumbung	≤-18 ℃
urip beting	12 sasi
Tipe Spesimen	sputum
CV	≤5,0%
LoD	LoD referensi nasional mycobacterium tuberculosis yaiku 50 bakteri / mL.LoD referensi nasional tipe liar tahan rifampisin yaiku 2×10³bakteri / mL, lan LoD jinis mutan yaiku 2 × 10³bakteri/mL.LoD bakteri tahan isoniazid jinis liar yaiku 2x10³bakteri / mL, lan LoD bakteri mutan yaiku 2x10³bakteri/mL.
Kekhususan	Asil tes silang nuduhake yen ora ana reaksi silang ing deteksi genom manungsa, mycobacteria non tuberkulosis liyane lan patogen pneumonia karo kit iki;Ora ana reaksi silang sing dideteksi ing situs mutasi gen tahan obat liyane ing Mycobacterium tuberculosis jinis liar.
Instrumen sing ditrapake	Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.), Sistem PCR BioRad CFX96 Real-Time, Teknologi Bioer Hangzhou QuantGene 9600 Sistem PCR Real-Time, QuantStudio^®5 Sistem PCR Real-Time.