Influenza A/B
jeneng produk
Kit Deteksi Asam Nukleat HWTS-RT003A Influenza A/B (PCR Fluoresensi)
Epidemiologi
Virus influenza A minangka lelara infèksi saluran napas akut, kanthi sawetara subtipe kayata H1N1 lan H3N2, sing rawan mutasi lan nyebar ing saindenging jagad.Pergeseran antigen nuduhake mutasi virus influenza A, sing ndadékaké munculé subtipe anyar.Virus influenza B dipérang dadi rong garis keturunan utama, Yamagata lan Victoria.Virus influenza B mung nduweni drift antigen, lan bisa nyingkiri pengawasan lan ngresiki sistem kekebalan manungsa liwat mutasi.Nanging, tingkat evolusi virus influenza B luwih alon tinimbang virus influenza A, lan virus influenza B uga bisa nyebabake infeksi saluran pernapasan manungsa lan nyebabake epidemi.
Saluran
| FAM | IFV A |
| ROX | Kontrol internal |
| VIC/HEX | IFV B |
Parameter teknis
| Lumbung | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | swab orofaringeal |
| Ct | ≤28 |
| CV | ≤5,0% |
| LoD | IFV A: 500 Salinan/mL, IFV B: 500 Salinan/mL |
| Kekhususan | 1. Reaktivitas silang: ora ana reaksi silang antarane kit iki lan adenovirus tipe 3, 7, coronavirus manungsa SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, lan HCoV-NL63, cytomegalovirus, enterovirus, virus parainfluenza, virus campak, metapneumovirus manungsa, virus gondok, virus respiratory syncytial tipe B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraxella catarrhalis neiculosis, myoplasma catarrhalis, myoplasma catarrhalis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius lan DNA genomik manungsa. 2. Tes interferensi: Zat interferensi dipilih minangka mucin (60mg/mL), getih manungsa, oxymetazoline (2mg/mL), sulfur (10%), beclomethasone (20mg/mL), deksametason (20mg/mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), mentol (10%), zanamivir (20mg/mL). ), azitromisin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir fosfat (60ng/mL), ribavirin (10mg/L), lan asil nuduhake yen zat sing ngganggu konsentrasi ing ndhuwur ora duwe reaksi ngganggu kanggo deteksi kit. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sistem PCR Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time lan Sistem PCR BioRad CFX Opus 96 Real-Time |
Alur Kerja
Pilihan 1.
Reagen ekstraksi sing disaranake: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Ekstraktor Asam Nukleat Otomatis (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut IFU.Volume sampel ekstraksi yaiku200 μL.Volume elusi sing disaranake yaiku 80μL.
Pilihan 2.
Reagen ekstraksi sing disaranake: Reagen Pelepasan Sampel Macro & Tes Mikro (HWTS-3005-8).Ekstraksi kudu ditindakake miturut IFU.
Pilihan 3.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315-R).Ekstraksi kudu ditindakake miturut IFU.Volume sampel ekstraksi yaiku 140μL.Volume elusi sing disaranake yaiku 60μL.
