Virus Influenza beku-kering/Virus Influenza B Asam Nukleat
jeneng produk
HWTS-RT193-Freeze-dried Influenza Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluoresensi PCR)
Epidemiologi
Miturut beda antigen antarane gen NP lan M, virus influenza bisa dipérang dadi papat jinis: virus influenza A (IFV A), virus influenza B (IFV B), virus influenza C (IFV C) lan virus influenza D (IFV D). Kanggo virus influenza A, virus iki nduweni akeh inang lan serotipe kompleks, lan bisa nyebar liwat inang liwat rekombinasi genetik lan mutasi adaptif. Manungsa ora duwe kekebalan sing tahan kanggo virus influenza A, mula wong-wong saka kabeh umur umume rentan. Virus influenza A minangka patogen paling utama sing nyebabake pandemi influenza. Kanggo virus influenza B, umume nyebar ing wilayah cilik lan saiki ora duwe subtipe. Infeksi manungsa utamane disebabake dening virus influenza saka garis keturunan B/Yamagata utawa B/Victoria. Antarane kasus influenza sing dikonfirmasi saben wulan ing 15 negara ing wilayah Asia-Pasifik, tingkat diagnosis virus influenza B antara 0 nganti 92%. Beda karo virus influenza A, klompok wong tartamtu, kayata bocah-bocah lan wong tuwa, rentan marang virus influenza B, sing bisa nyebabake komplikasi kanthi gampang, malah ndadekake beban masyarakat luwih akeh tinimbang virus influenza A.
Parameter teknis
| Panyimpenan | 2-28℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | Sapuan tenggorokan |
| Ct | IFV A,IFVB Ct≤35 |
| CV | <5.0% |
| LoD | 200 eksemplar/mL |
| Kekhususan | Reaktivitas silang: Ora ana reaktivitas silang ing antarane kit lan Bocavirus, Rhinovirus, Cytomegalovirus, Respiratory syncytial virus, Parainfluenza virus, Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, Mumps virus, Enterovirus, Measles virus, human metapneumovirus, coronavirus Human metapneumovirus, Adenovirus, MV, coronavirus Rotavirus, Norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis jirovecii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberkulosis, Candidosis Candida, Candidosis glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium lan DNA genomik manungsa. Tes interferensi: Mucin (60mg/mL), getih manungsa (50%), Phenylephrine (2 mg/mL), Oxymetazoline (2mg/mL), Natrium klorida (20mg/mL) kanthi pengawet 5%, Beclomethasone (20mg/mL), Dexamethasone (20mg/mL), Flunisoligide (20mg/mL), (2mg/mL), Budesonide (1mg/mL), Mometasone (2mg/mL), Fluticasone (2mg/mL), Histamine hydrochloride (5 mg/mL), Benzocaine (10%), Menthol (10%), Zanamivir (20mg/mL), Peramivir (1mg/mL), Mupiromcin (20mg/mL), 6mg/mL Oseltamivir (60ng / mL), Ribavirin (10mg / L) dipilih kanggo tes interferensi, lan asil kasebut nuduhake yen zat sing ngganggu ing konsentrasi ing ndhuwur ora duwe reaksi interferensi marang asil tes kit. |
| Instrumen sing ditrapake | Ditrapake kanggo reagen tes Tipe I: Applied Biosystems 7500 Sistem PCR Real-Time, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.). Ditrapake kanggo reagen tes Tipe II: EudemonTM AIO800 (HWTS-EQ007) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Alur Kerja
PCR konvensional
Kit DNA/RNA Umum Macro & Micro-Test (HWTS-3019) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Macro & Micro-Test (HWTS-3006C, (HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. disaranake kanggo ekstraksi sampel miturut langkah-langkah sing disaranake miturut langkah-langkah Kit FU.
AIO800 mesin kabeh-ing-siji
