28 Jinis Human Papilloma Virus Berisiko Tinggi (Pengetikan 16/18) Asam Nukleat
jeneng produk
HWTS-CC006A-28 Jinis Human Papilloma Virus Berisiko Tinggi (Pengetikan 16/18) Kit Deteksi Asam Nukleat (PCR Fluoresensi)
Epidemiologi
Kanker serviks minangka salah sawijining tumor ganas sing paling umum ing saluran reproduksi wanita.Panaliten nuduhake manawa infeksi HPV sing terus-terusan lan macem-macem infeksi minangka salah sawijining panyebab utama kanker serviks.Saiki, perawatan efektif sing diakoni isih kurang kanggo kanker serviks sing disebabake dening HPV, mula panemuan awal lan pencegahan infeksi serviks sing disebabake dening HPV minangka kunci kanggo nyegah kanker serviks.Penting banget kanggo nggawe tes diagnostik etiologi sing prasaja, spesifik lan cepet kanggo diagnosa klinis lan perawatan kanker serviks.
Saluran
| Campur Reaksi | Saluran | Jinis |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Kontrol internal | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Kontrol internal |
Parameter teknis
| Lumbung | Cairan: ≤-18 ℃ |
| urip beting | 12 sasi |
| Tipe Spesimen | Serviks Swab, Vagina Swab, Urine |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 eksemplar / ml |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 200μL saline normal kanggo resuspensi pelet ing langkah 2.1, banjur ekstraksi kudu ditindakake miturut kanggo pandhuan kanggo nggunakake reagen ekstraksi iki.Volume elusi sing disaranake yaiku 80μL.
Reagen ekstraksi sing disaranake: QIAamp DNA Mini Kit (51304) utawa Kolom DNA/RNA Viral Macro & Micro-Test (HWTS-3020-50).Tambah 200μL saline normal kanggo resuspend pelet ing langkah 2.1, lan banjur extraction kudu conducted miturut pandhuan kanggo nggunakake reagen extraction iki.Volume sampel sing diekstrak kabeh 200μL, lan volume elusi sing disaranake yaiku 100μL.
