Gabungan 14 Jinis Patogen Pernapasan
Jeneng produk
HWTS-RT159B 14 Jinis Patogen Pernapasan Kit Deteksi Asam Nukleat Gabungan (PCR Fluoresensi)
Epidemiologi
Infeksi saluran pernapasan minangka penyakit sing paling umum ing manungsa, sing bisa kedadeyan ing kabeh jinis, umur, lan wilayah. Iki minangka salah sawijining panyebab utama morbiditas lan mortalitas ing populasi ing saindenging jagad.[1]Patogen pernapasan umum kalebu novel coronavirus, virus Influenza A, virus Influenza B, virus Respiratory syncytial, Adenovirus, human metapneumovirus, rhinovirus, virus Parainfluenza tipe I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, lan Streptococcus pneumoniae, lan liya-liyane.[2,3].
Saluran
| Posisi Sumur | Jeneng Larutan Reaksi | Patogen sing kudu dideteksi |
| 1 | Campuran Master A | SARS-CoV-2, IFV A, IFV B |
| 2 | Campuran Master B | Adv, hMPV, MP, Cpn |
| 3 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, kontrol internal |
| 5 | Campuran Master A | SARS-CoV-2, IFV A, IFV B |
| 6 | Campuran Master B | Adv, hMPV, MP, Cpn |
| 7 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, kontrol internal |
Parameter Teknis
| Panyimpenan | ≤-18℃ |
| Umur simpan | 9 sasi |
| Jinis Spesimen | Swab orofaringeal, swab nasofaring |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 Salinan/mL |
| Spesifisitas | Asil uji reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit iki lan Cytomegalovirus, virus Herpes simplex tipe 1, virus Varicella-zoster, virus Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, galur Mycobacterium tuberculosis sing dilemahake, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii lan asam nukleat genomik manungsa. |
| Instrumen sing Ditrapake | Sistem PCR Wektu Nyata SLAN-96P (Hongshi Medical Technology Co., Ltd.)Sistem PCR Wektu Nyata Biosistem Terapan 7500 Sistem PCR Wektu Nyata Cepet Biosistem Terapan 7500 QuantStudio®5 Sistem PCR Wektu Nyata Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Hangzhou Bioer) Siklus Termal Kuantitatif Wektu Nyata MA-6000 (Suzhou Molarray Co., Ltd.) Sistem PCR Wektu Nyata BioRad CFX96, Sistem PCR Wektu Nyata BioRad CFX Opus 96 |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Macro & Micro-Test (HWTS-EQ010)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volume sampel sing diekstrak yaiku 200µL. Langkah-langkah sabanjure kudu ditindakake miturut pandhuan kanggo nggunakake reagen ekstraksi iki. Volume elusi sing disaranake yaiku80µL.
