14 Jinis Patogen Pernafasan Gabungan
jeneng produk
HWTS-RT159B 14 Jinis Kit Deteksi Asam Nukleat Gabungan Patogen Pernafasan (PCR Fluoresensi)
Epidemiologi
Infeksi saluran pernapasan minangka penyakit sing paling umum ing manungsa, sing bisa kedadeyan ing kabeh jinis, umur lan wilayah. Iki minangka salah sawijining panyebab utama morbiditas lan mortalitas ing populasi ing saindenging jagad[1]. Patogen pernapasan sing umum kalebu coronavirus novel, virus Influenza A, virus Influenza B, Virus syncytial Respiratory, Adenovirus, metapneumovirus manungsa, rhinovirus, virus Parainfluenza tipe I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, lan Streptococcus pneumoniae, lsp.[2,3].
Saluran
| Posisi Inggih | Jeneng Solusi Reaksi | Patogen sing bakal dideteksi |
| 1 | Master Campuran A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Campuran B | Adv, hMPV, MP, Cpn |
| 3 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Campuran D | CoV, EV, SP, kontrol internal |
| 5 | Master Campuran A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Campuran B | Adv, hMPV, MP, Cpn |
| 7 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Campuran D | CoV, EV, SP, kontrol internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | Swab oropharyngeal, swab nasofaring |
| Ct | ≤38 |
| CV | <5,0% |
| LoD | 200 eksemplar/mL |
| Kekhususan | Asil tes reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit iki lan Cytomegalovirus, virus Herpes simplex tipe 1, virus Varicella-zoster, virus Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilalis saka galur. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia, No. Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii lan inti genomik asam manungsa. |
| Instrumen sing ditrapake | Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.)Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time, Sistem PCR BioRad CFX Opus 96 Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Macro & Micro-Test (HWTS-EQ010)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volume sampel sing diekstrak yaiku 200µL. Langkah-langkah sabanjure kudu ditindakake miturut pandhuan kanggo nggunakake reagen ekstraksi iki. Volume elusi sing disaranake yaiku80µL.
