Virus Demam Hemoragik Xinjiang
jeneng produk
HWTS-FE007B/C Xinjiang Hemorrhagic Fever Virus Nucleic Acid Detection Kit (Fluoresensi PCR)
Epidemiologi
Virus demam hemoragik Xinjiang pisanan diisolasi saka getih pasien demam berdarah ing Cekungan Tarim, Xinjiang, China lan kutu keras sing ditangkep sacara lokal, lan entuk jenenge. Manifestasi klinis kalebu mriyang, sirah, getihen, kejut hipotensi, lan liya-liyane. Owah-owahan patologis dhasar saka penyakit iki yaiku dilatasi kapiler sistemik, kemacetan, tambah permeabilitas lan fragility, sing nyebabake macem-macem tingkat kemacetan lan pendarahan ing kulit lan membran mukus uga jaringan saka macem-macem organ ing awak, kanthi degenerasi lan kelenjar adrenal, kelenjar adrenal, lan liya-liyane. edema kaya jeli ing retroperitoneum.
Saluran
| FAM | Virus Demam Hemoragik Xinjiang |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | serum seger |
| Tt | ≤38 |
| CV | <5,0% |
| LoD | 1000 eksemplar / mL |
| Kekhususan | Ora ana reaksi silang karo conto pernapasan liyane kayata Influenza A, Influenza B, Legionella pneumophila, demam Rickettsia Q, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/Bncytial virus, Metapneumovirus A1/Bncytial virus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, virus Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, lan sapiturute lan DNA genomik manungsa. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time, Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Extractor Asam Nukleat Otomatis Makro & Mikro-Test (HWTS-EQ011)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut instruksi ekstraksi iki. Volume sampel sing diekstrak yaiku 200µL, lan volume elusi sing disaranake yaiku 80µL.
Reagen ekstraksi sing disaranake: QIAamp Viral RNA Mini Kit (52904) dening QIAGEN lan Reagen Ekstraksi utawa Pemurnian Asam Nukleat (YDP315-R). Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake. Volume sampel sing diekstraksi yaiku 140μL, lan volume elusi sing disaranake yaiku 60μL.
