Staphylococcus Aureus lan Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
jeneng produk
HWTS-OT062 Staphylococcus Aureus lan Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Kit Deteksi Asam Nukleat (Fluoresensi PCR)
Sertifikat
CE
Epidemiologi
Staphylococcus aureus minangka salah sawijining bakteri patogen penting infeksi nosokomial.Staphylococcus aureus (SA) kalebu staphylococcus lan minangka wakil saka bakteri Gram-positif, sing bisa ngasilake macem-macem racun lan enzim invasif.Bakteri kasebut nduweni karakteristik distribusi sing wiyar, patogenisitas sing kuat lan tingkat resistensi sing dhuwur.Gen nuklease termostabil (nuc) minangka gen staphylococcus aureus sing dikonservasi banget.
Saluran
| FAM | gen mecA tahan methicillin |
| ROX | Kontrol internal |
| CY5 | gen staphylococcus aureus nuc |
Parameter teknis
| Lumbung | ≤-18 ℃ & dilindhungi saka cahya |
| urip beting | 12 sasi |
| Tipe Spesimen | sputum, sampel infeksi kulit lan jaringan alus, lan sampel swab irung |
| Ct | ≤36 |
| CV | ≤5,0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bakteri tahan methicillin.Nalika kit ndeteksi referensi LoD nasional, 1000/mL staphylococcus aureus bisa dideteksi |
| Kekhususan | Tes reaktivitas silang nuduhake yen kit iki ora duwe reaktivitas silang karo patogen pernapasan liyane kayata staphylococcus aureus sing sensitif methicillin, staphylococcus coagulase-negatif, staphylococcus epidermidis sing tahan methicillin, pseudomonas aeruginosa, escherichia coli, klebsiumaniella, pneumoniae mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Tambah 200μL saline normal menyang endapan sing diproses, lan langkah-langkah sabanjure kudu diekstrak miturut instruksi, lan volume elusi sing disaranake yaiku 80μL.
Pilihan 2.
Reagen Pelepasan Sampel Macro & Micro-Test (HWTS-3005-8) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 1mL saline normal menyang endapan sawise dicuci nganggo saline normal, banjur aduk kanthi becik.Centrifuge ing 13.000r / min kanggo 5 menit, copot supernatant (cadangan 10-20μL supernatant), lan tindakake pandhuan kanggo ekstraksi sakteruse.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP302) dening Tiangen Biotech (Beijing) Co.,Ltd.Ekstraksi kudu ditindakake kanthi ketat miturut langkah 2 saka instruksi manual.Disaranake nggunakake banyu bebas RNase lan DNase kanggo elusi kanthi volume 100µL.
