Orientia tsutsugamushi
jeneng produk
HWTS-OT002B Orientia tsutsugamushiKit Deteksi Asam Nukleat (PCR Fluoresensi)
Epidemiologi
Scrub typhus yaiku penyakit demam akut sing disebabake dening infeksi Orientia tsutsugamushi (Ot). Orientia scrub typhus minangka mikroorganisme parasit intraselular obligat Gram-negatif. Orientia scrub typhus kalebu genus Orientia ing ordo Rickettsiales, kulawarga Rickettsiaceae, lan genus Orientia. Scrub typhus utamane ditularake liwat gigitan larva chigger sing nggawa patogen. Klinis ditondoi kanthi demam dhuwur dadakan, eschar, limfadenopati, hepatosplenomegali, lan leukopenia getih perifer, lan liya-liyane. Ing kasus sing abot, bisa nyebabake meningitis, gagal ati lan ginjel, gagal multi-organ sistemik, lan malah mati.
Saluran
| FAM | Orientia tsutsugamushi |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | serum seger |
| Tt | ≤38 |
| CV | ≤5,0% |
| LoD | 500 eksemplar/μL |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sistem PCR Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time, Sistem PCR BioRad CFX Opus 96 Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Tes Makro & MikroUmumKit DNA/RNA (HWTS-3019) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut instruksi kanggo nggunakake reagen ekstraksi iki. Volume sampel sing diekstrak yaiku 200µL, lan volume elusi sing disaranake yaiku100µL.
