Orientia tsutsugamushi Asam Nukleat
jeneng produk
HWTS-OT002-Orientia tsutsugamushi Kit Deteksi Asam Nukleat (Fluoresensi PCR)
Epidemiologi
Scrub typhus minangka penyakit demam akut sing disebabake dening infeksi Orientia tsutsugamushi (Ot). Orientia scrub typhus minangka mikroorganisme parasit intraselular obligat Gram-negatif. Orientia scrub typhus kalebu genus Orientia ing ordo Rickettsiales, kulawarga Rickettsiaceae, lan genus Orientia. Scrub typhus utamane ditularake liwat gigitan larva chigger sing nggawa patogen. Klinis ditondoi kanthi demam dhuwur dadakan, eschar, limfadenopati, hepatosplenomegali, lan leukopenia getih perifer, lan liya-liyane. Ing kasus sing abot, bisa nyebabake meningitis, gagal ati lan ginjel, gagal multi-organ sistemik, lan malah mati.
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | sampel serum |
| CV | ≤5,0% |
| LoD | 500 eksemplar/μL |
| Instrumen sing ditrapake | Ditrapake kanggo reagen deteksi tipe I: Applied Biosystems 7500 Sistem PCR Real-Time, QuantStudio®5 Sistem PCR Real-Time, Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Sistem Deteksi PCR Wektu Nyata (FQD-96A, teknologi Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistem PCR BioRad CFX96 Real-Time, BioRad CFX Opus 96 Sistem PCR Real-Time. Ditrapake kanggo reagen deteksi tipe II: EudemonTMAIO800 (HWTS-EQ007) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Alur Kerja
Makro & Micro-Test Umum DNA / RNA Kit (HWTS-3019) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu dilakoni miturut instruksi kanggo nggunakake reagen ekstraksi iki. Volume sampel sing diekstrak yaiku 200μL, lan volume elusi sing disaranake yaiku 100μL.
