Mycoplasma Pneumoniae (MP)
jeneng produk
HWTS-RT024 Mycoplasma Pneumoniae（MP） Kit Deteksi Asam Nukleat(Fluoresensi PCR)
Epidemiologi
Mycoplasma pneumoniae (MP) minangka jinis mikroorganisme prokariotik sing paling cilik, yaiku antarane bakteri lan virus, kanthi struktur sel nanging ora ana tembok sel.MP utamane nyebabake infeksi saluran pernapasan manungsa, utamane ing bocah-bocah lan wong enom.Bisa nyebabake radhang paru-paru mycoplasma manungsa, infeksi saluran pernapasan bocah lan pneumonia atipikal.Manifestasi klinis macem-macem, sing paling akeh yaiku batuk parah, mriyang, hawa adhem, sirah, lara tenggorokan.Infeksi saluran napas ndhuwur lan pneumonia bronkial sing paling umum.Sawetara pasien bisa ngalami infeksi saluran pernapasan ndhuwur nganti radhang paru-paru sing abot, gangguan pernapasan sing abot lan bisa uga mati.
Saluran
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Kontrol internal |
Parameter teknis
| Lumbung | ≤-18 ℃ |
| urip beting | 12 sasi |
| Tipe Spesimen | Sputum, swab orofaringeal |
| Ct | ≤38 |
| CV | ≤5,0% |
| LoD | 200 eksemplar/mL |
| Kekhususan | a） Reaktivitas silang: ora ana reaktivitas silang karo Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella ba. virus influenza A , Virus Influenza B, Virus Parainfluenza tipe I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus lan human genomic nucleic acid. b）Kemampuan anti-interferensi: ora ana interferensi nalika zat sing ngganggu diuji kanthi konsentrasi ing ngisor iki: hemoglobin (50mg/L), bilirubin (20mg/dL), musin (60mg/mL), 10% (v/v) getih manungsa, levofloxacin (10μg/mL), moxifloxacin (0,1g/L), gemifloxacin (80μg/mL), azitromisin (1mg/mL), klaritromisin (125μg/mL), eritromisin (0,5g/L), doksisiklin (50mg). /L), minocycline (0,1g/L). |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sistem PCR Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
(1) Sampel sputum
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Ekstraktor Asam Nukleat Otomatis (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 200µL saline normal menyang endapan sing diproses.Ekstraksi sabanjure kudu ditindakake miturut pandhuan kanggo nggunakake.Volume elusi sing disaranake yaiku 80µL. Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP315-R).Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake.Volume elusi sing disaranake yaiku 60µL.
(2) Swab orofaringeal
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut pandhuan kanggo nggunakake.Volume ekstraksi sing disaranake yaiku 200µL, lan volume elusi sing disaranake yaiku 80µL. Reagen ekstraksi sing disaranake: Kit Mini RNA Viral QIAamp (52904) utawa Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP315-R).Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake.Volume ekstraksi sing disaranake yaiku 140μL, lan volume elusi sing disaranake yaiku 60μL.
