Peumoniae MyCoplasma (MP)
Jeneng Produk
HWTTS-RT024 MyCoplasma Pneumoniae (MP) Kit deteksi asam nukleat (PCR Fluoresescence)
Epidemiologi
Peumoniae MyCoplasma (MP) minangka jinis mikroorganisme prokaryotik paling cilik, yaiku antarane bakteri lan virus, kanthi struktur sel nanging ora ana tembok sel. MP utamane nyebabake infeksi saluran pernapasan manungsa, utamane ing bocah-bocah lan wong enom. Bisa nyebabake radhang paru-paru mycoplasma, infeksi pernapasan pernapukan bocah lan radhang paru-paru atypical. Manifestasi klinis yaiku macem-macem, sing paling akeh batuk, demam, nyegel, nyeri sirah, sakit tenggorokan. Insibeksi saluran pernap ndhuwur lan radhang paru-paru bronchial paling umum. Sawetara pasien bisa berkembang saka infeksi saluran pernap ndhuwur ndhuwur kanggo radhang paru-paru abot, kasusahan ambegan abot, kasusahan ambegan sing abot bisa kedadeyan.
Saluran
| Fames | Pneumoniae MyCoplasma |
| Vic / Hex | Kontrol internal |
Paramèter teknis
| Panyimpen | ≤-18 ℃ |
| Beting urip | 12 wulan |
| Jinis spesimen | Sputum, Oropharyngeal Swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 200 salinan / ml |
| Kekhususan | a) reaktivitas salib: ora ana reaktivitas salib karo ureaplasma urentivum, mycoplasma genitalium, pneumoniae Klokisma, staphylococcus aureus, tuberkolosis mycobactus, legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. b) kemampuan anti-gangguan: ora ana gangguan nalika bahan-bahan campur tangan dites karo konsentrasi ing ngisor iki: hemoglobin (50mg / dl), mucin (60mg / ml), 10% (v / v) Getih manungsa, levofloxacin (10μg / ml), moxifloxacin (0.1g / l), gemifloxacin (80μg / ml), azithromycin (1mg / ml), clarithromycin (125μg / l), doxycycline (50mg / l), 0.1g / l). |
| Instrumen sing ditrapake | Biosistem Applied 7500 sistem PCR nyata-wektu Biosistem Applied 7500 Sistem PCR wektu nyata-wektu cepet Quantstudio®5 Sistem PCR nyata-wektu SLAN-96P System PCR-Time-Time (Technology Technology HONGSHI CH, Ltd) Lightcycler®480 Sistem PCR nyata-wektu Plancene 9600 Plus sistem deteksi PCR nyata-wektu (FQD-96A, teknologi bioer Hangzhou) MA-6000 Masmal Cupter Thermal Real-Time-Time-Time-Time-Time (Suzhou Molarray Co, Ltd) Sistem PCR nyata-wektu Biorad CFX96 Sistem PCR nyata-wektu 96 |
Aliran kerja
(1) Sampel Sputum
Reagent Extraction Disaranake: Macro & Micro-Tes Viry DNA / RNA Kit (HWTS-3017-48, sing bisa digunakake karo Macro & Micro-Test Ekstrak asam nukleik otomatis (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co, Ltd. Tambah 200-200-ilmu normal kanggo olahan ecep. Ekstraksi sakteruse kudu ditindakake miturut pitunjuk sing dienggo. Volume pencegahan sing disaranake yaiku reagen ekstraksi 80μL.Recommended: ekstraksi asam nukleat utawa reagen purifikasi (ydp315-r). Ekstraksi kudu ditindakake miturut instruksi kanggo nggunakake. Volume volume sing disaranake yaiku 60μm.
(2) swab oropharyngeal
Reagent Extraction Disaranake: Macro & Micro-Tes Viry DNA / RNA Kit (HWTS-3017-48, sing bisa digunakake karo Macro & Micro-Test Ekstrak asam nukleik otomatis (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test med-Tech Med-Tech Co, Ltd ekstraksi kudu ditindakake miturut pitunjuk kanggo nggunakake. Tolahan ekstraksi sing disaranake yaiku 200μL, lan volume pencegahan sing disaranake yaiku 80 -L.Ractors Reagent: Qiaamp Viral RNA Mini Kit (52904) utawa reagen asam nukléah (YDP315-R). Ekstraksi kudu ditindakake miturut instruksi kanggo nggunakake. Tombol ekstraksi sing disaranake yaiku 140μl, lan volume volume sing disaranake yaiku 60μ.
