Virus Influenza A Universal/H1/H3
jeneng produk
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluoresensi PCR)
Epidemiologi
Virus influenza minangka spesies perwakilan Orthomyxoviridae. Iki minangka patogen sing mbebayani banget kanggo kesehatan manungsa. Bisa nginfèksi inang sacara ekstensif. Epidemi musiman nyebabake kira-kira 600 yuta wong ing saindenging jagad lan nyebabake 250.000 ~ 500.000 tiwas, sing virus influenza A minangka panyebab utama infeksi lan pati. Virus influenza A minangka RNA untai negatif untai tunggal. Miturut hemagglutinin permukaan (HA) lan neuraminidase (NA), HA bisa dipérang dadi 16 subtipe, NA Dipérang dadi 9 subtipe. Ing antarane virus influenza A, subtipe virus influenza sing bisa langsung nginfeksi manungsa yaiku: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 lan H10N8. Antarane wong-wong mau, subtipe H1 lan H3 banget pathogenic, lan utamané pantes manungsa waé.
Saluran
| FAM | influenza A virus tipe universal asam nukleat |
| VIC/HEX | influenza A H1 virus asam nukleat |
| ROX | influenza A H3 virus asam nukleat |
| CY5 | kontrol internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | swab nasofaring |
| Ct | ≤38 |
| CV | ≤5,0% |
| LoD | 500 eksemplar/μL |
| Kekhususan | Ora ana reaksi silang karo conto pernapasan liyane kayata Influenza A, Influenza B, Legionella pneumophila, demam Rickettsia Q, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/Bncytial virus, Metapneumovirus A1/Bncytial virus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, virus Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, lan sapiturute lan DNA genomik manungsa. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P(Hongshi Medical Technology Co., Ltd.) Sistem PCR LightCycler®480 Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP315-R) dening Tiangen Biotech (Beijing) Co.,Ltd. Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake. Volume sampel sing diekstrak yaiku 140μL, lan volume elusi sing disaranake yaiku 60μL.
Pilihan 2.
Kit DNA/RNA Viral Uji Makro & Mikro (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) lan Ekstraktor Asam Nukleat Otomatis Makro & Uji Mikro (HWTS-3006C, HWTS-3006B). Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake. Volume sampel sing diekstrak yaiku 200μL, lan volume elusi sing disaranake yaiku 80μL.
