Virus Influenza A/ Virus Influenza B
jeneng produk
HWTS-RT174-Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluoresensi PCR)
Epidemiologi
Adhedhasar prabédan antigen antarané gen NP lan gen M, virus influenza bisa dipérang dadi patang jinis: virus influenza A (IFV A), virus influenza B (IFV B), virus influenza C (IFV C) lan virus influenza D (IFV D).[1]. Virus influenza A nduweni akeh host lan serotipe kompleks, lan bisa entuk kemampuan kanggo nyebar liwat host liwat rekombinasi genetik lan mutasi adaptif. Manungsa ora duwe kekebalan sing tahan kanggo virus influenza A, mula wong-wong saka kabeh umur umume rentan. Virus influenza A minangka patogen utama sing nyebabake pandemi influenza[2]. Virus influenza B umume nyebar ing wilayah cilik lan saiki ora duwe subtipe. Sing utama sing nyebabake infeksi manungsa yaiku garis keturunan B/Yamagata utawa garis keturunan B/Victoria. Antarane kasus influenza sing dikonfirmasi ing 15 negara ing wilayah Asia-Pasifik saben wulan, tingkat virus influenza B sing dikonfirmasi yaiku 0-92%[3]. Ora kaya virus influenza A, kelompok tartamtu kayata bocah-bocah lan wong tuwa rentan kena virus influenza B lan rentan ngalami komplikasi, sing ndadekake beban masyarakat luwih gedhe tinimbang virus influenza A.[4].
Saluran
| FAM | asam nukleat MP |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | Sampel swab orofaringeal |
| Ct | Flu A, Flu BCt≤35 |
| CV | <5.0% |
| LoD | Flu A lan Flu Bkabeh 200 Salinan / mL |
| Kekhususan | Reaktivitas silang: Ora ana reaksi silang antarane kit lan Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, virus parainfluenza, virus Epstein-Barr, virus herpes simplex, virus Varicella-zoster, virus mumps, enterovirus, virus campak, metapneumovirus manungsa, adenovirus, coronavirus manungsa, virus corona virus MERSro, virus corona virus, virus corona virus, MERSro coronavirus pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeanae, Candida sp. Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, lan DNA genom manungsa. Tes interferensi: Pilih musin (60 mg/mL), getih manungsa (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), natrium klorida (20mg/mL) kanthi pengawet 5%, beclomethasone (20mg/mL), deksametason (20mg/mL), flunisoligide (20mg/mL), a. (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), flutikason (2mg/mL), histamin hidroklorida (5mg/mL), benzocaine (10%), mentol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpiromL (20mg/L), oseltamivir (60ng/mL), ribavirin (10mg/L) kanggo tes interferensi, lan asil nuduhake yen zat sing ngganggu ing konsentrasi ing ndhuwur ora ngganggu deteksi kit. |
| Instrumen sing ditrapake | Sistem PCR Real-Time SLAN-96P Applied Biosystems 7500 Real-Time PCR System QuantStudio®5 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (sing bisa digunakake karo Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.dianjurake kanggo ekstraksi sampel lanlangkah sakteruse kudukondukted ing selaras ketat karo IFUsaka Kit.
