Manungsa PML-RARA Fusion Gene Mutation
jeneng produk
HWTS-TM017AKit Deteksi Mutasi Gene Fusion PML-RARA Manungsa (PCR Fluoresensi)
Epidemiologi
Leukemia promyelocytic akut (APL) yaiku jinis khusus saka leukemia mieloid akut (AML). Kira-kira 95% pasien APL diiringi owah-owahan sitogenetik khusus, yaiku t(15;17)(q22;q21), sing ndadekake gen PML ing kromosom 15 lan gen reseptor asam retinoat α (RARA) ing kromosom 17 digabung dadi gen fusi PML-RARA. Amarga breakpoints gen PML sing beda, gen fusi PML-RARA bisa dipérang dadi jinis dawa (tipe L), jinis cendhak (tipe S) lan jinis varian (tipe V), kira-kira 55%, 40% lan 5%.
Saluran
| FAM | Gen fusi PML-RARA |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ Ing peteng |
| urip beting | 9 wulan |
| Tipe Spesimen | sumsum balung |
| CV | <5.0％ |
| LoD | 1000 eksemplar/mL. |
| Kekhususan | Ora ana reaktivitas silang karo gen fusi liyane BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, lan gen fusi TEL-AML1 |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sistem PCR Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Bioer Hangzhou) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit Ekstraksi RNA Total Darah Murni RNAprep (DP433). Ekstraksi kudu ditindakake miturut IFU.
