Mutasi Gene Gene Gene Manungsa PML-Rara Fusion
Jeneng Produk
Hwts-tm017AHuman Gen Deteksi Deteksi Kit (PCR Fluoresescence)
Epidemiologi
Leukemia promyelostic akut (APL) minangka jinis khusus akut myelemia (AML). About 95% of APL patients are accompanied by a special cytogenetic change, namely t(15;17)(q22;q21), which makes the PML gene on chromosome 15 and the retinoic acid receptor α gene (RARA) on chromosome 17 fused to mbentuk Gene Gene PML-Rara Fusion. Amarga breakpour saka Gene PLL, Gene PML-Rara Fusion bisa dipérang dadi jinis sing dawa (L (jinis jinis), jinis varian (Varian kira-kira 55%, 40% lan 5 % masing-masing.
Saluran
| Fames | Gene Gene Fusion Pml-Rara |
| Rok | Kontrol internal |
Paramèter teknis
| Panyimpen | ≤-18 ℃ ing peteng |
| Beting urip | 9 wulan |
| Jinis spesimen | sumsum balung |
| CV | <5.0% |
| Lod | 1000 salinan / ml. |
| Kekhususan | Ora ana reaktivity salib karo gen bcr-abl liyane, E2A-PBX1, MLL-AF4, AML1-ETO, lan Geneng Tel-Aml1 Fusion |
| Instrumen sing ditrapake | Biosistem Applied 7500 sistem PCR nyata-wektu Biosistem Applied 7500 Sistem PCR wektu nyata-wektu cepet Quantstudio®5 Sistem PCR nyata-wektu SLAN-96P System PCR-Time-Time (Technology Technology HONGSHI CH, Ltd) Lightcycler®480 Sistem PCR nyata-wektu Plancene 9600 Plus sistem deteksi PCR nyata-wektu (FQD-96A, teknologi bioer Hangzhou) MA-6000 Masmal Cupter Thermal Real-Time-Time-Time-Time-Time (Suzhou Molarray Co, Ltd) Sistem PCR nyata-wektu Biorad CFX96 Sistem PCR nyata-wektu 96 |
Aliran kerja
Reagent Extraction Disaranake: RNArepep murni ekstraksi total getih total (DP433). Ekstraksi kudu ditindakake miturut IFU.
