Virus Hepatitis A
jeneng produk
HWTS-HP005 Kit Deteksi Asam Nukleat Virus Hepatitis A (Fluoresensi PCR)
Epidemiologi
Virus Hepatitis A (HAV) minangka penyebab utama hepatitis virus akut.Virus iki minangka virus RNA untaian tunggal sing duwe rasa positif lan kalebu genus Hepadnavirus saka kulawarga Picornaviridae.Virus Hepatitis A, utamane ditularake liwat fecal-oral, tahan panas, asam, lan umume pelarut organik, bisa tahan suwe ing kerang, banyu, lemah, utawa sedimen dhasar laut [1-3].Iki ditularake kanthi panganan utawa banyu sing kontaminasi, utawa langsung ditularake saka wong menyang wong.Panganan sing ana gandhengane karo HAV kalebu tiram lan kerang, stroberi, raspberry, blueberry, kurma, sayuran godhong ijo, lan tomat semi-garing [4-6].
Saluran
| FAM | asam nukleat HAV |
| ROX | Kontrol internal |
Parameter teknis
| Lumbung | ≤-18 ℃ |
| urip beting | Cairan: 9 sasi, Lyophilized: 12 sasi |
| Tipe Spesimen | Serum / bangku |
| Tt | ≤38 |
| CV | ≤5,0% |
| LoD | 2 eksemplar/μL |
| Kekhususan | Gunakake kit kanggo nguji virus hepatitis liyane kayata hepatitis B, C, D, E, enterovirus 71, virus coxsackie, virus Epstein-Barr, norovirus, HIV lan genom manungsa. Ora ana reaksi silang. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P(Hongshi Medical Technology Co., Ltd.) Sistem PCR LightCycler®480 Real-Time LineGene 9600 Plus Sistem Deteksi PCR Wektu Nyata (FQD-96A, teknologi Bioer Hangzhou), MA-6000 Real-Time Quantitative Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Sampel serum
Pilihan 1.
Kit DNA/RNA Umum Uji Makro & Mikro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) lan Ekstraktor Asam Nukleat Otomatis Makro & Uji Mikro (HWTS-3006C, HWTS-3006B).Sampeyan kudu diekstrak miturut pandhuane.Volume elusi sing disaranake yaiku 80µL.
Pilihan 2.
Kit DNA/RNA Virus TIANamp (YDP315-R) diprodhuksi dening Tiangen Biotech (Beijing) Co., Ltd. Sampeyan kudu diekstrak miturut pandhuane.Volume sampel sing diekstrak yaiku 140μL.Volume elusi sing disaranake yaiku 60µL.
2.Sampel bangku
Kit DNA/RNA Umum Uji Makro & Mikro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) lan Ekstraktor Asam Nukleat Otomatis Makro & Uji Mikro (HWTS-3006C, HWTS-3006B).Sampeyan kudu diekstrak miturut pandhuane.Volume elusi sing disaranake yaiku 80µL.
