Nukleat Virus Hantaan
jeneng produk
HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluoresensi PCR)
Epidemiologi
Hantavirus minangka jinis virus RNA untai negatif, disegmentasi, disegmentasi. Hantavirus dipérang dadi rong jinis: siji nyebabake Hantavirus pulmonary syndrome (HPS), lan liyane nyebabake Hantavirus hemorrhagic fever with renal syndrome (HFRS). Tilas utamane umum ing Eropa lan Amerika Serikat, lan sing terakhir yaiku demam hemorrhagic kanthi sindrom ginjel sing disebabake dening virus Hantaan, sing umum ing China. Gejala hantavirus jinis hantaan utamané mujudaké mriyang hemorrhagic karo sindrom ginjel, kang ditondoi dening mriyang dhuwur, hypotension, getihen, oliguria utawa polyuria lan gangguan fungsi ginjel liyane. Iku pathogenic kanggo manungsa lan kudu mbayar manungsa waé sing cukup kanggo.
Saluran
| FAM | jinis hantavirus |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | serum seger |
| Ct | ≤38 |
| CV | <5,0% |
| LoD | 500 eksemplar/μL |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Macro & Micro-Test (HWTS-EQ011)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut IFU. Volume sampel ekstraksi yaiku 200μL. Volume elusi sing disaranake yaiku 80μL.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315-R). Ekstraksi kudu ditindakake miturut IFU. Volume sampel ekstraksi yaiku 140μL. Volume elusi sing disaranake yaiku 60μL.
