Forest Encephalitis Virus
jeneng produk
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit (Fluoresensi PCR)
Epidemiologi
Forest encephalitis (FE), uga dikenal minangka tick-borne encephalitis (Tick-borne encephalitis, TBE), yaiku penyakit infèksi akut saka sistem saraf pusat sing disebabake dening virus ensefalitis alas. Virus encephalitis alas kalebu genus Flavivirus saka kulawarga Flaviviridae. Partikel virus iku bunder kanthi diameter 40-50nm. Bobot molekul kira-kira 4 × 106Da, lan genom virus iku positif-rasa, RNA untai tunggal[1]. Secara klinis, ditondoi dening demam dhuwur, sirah, koma, iritasi meningeal kanthi cepet, lan paralisis otot ing gulu lan perangan awak, lan nduweni tingkat kematian sing dhuwur. Diagnosis awal lan cepet virus encephalitis alas minangka kunci kanggo perawatan encephalitis alas, lan panyiapan metode diagnosis etiologis sing prasaja, spesifik lan cepet iku penting banget ing diagnosis klinis encephalitis alas.[1,2].
Saluran
| FAM | asam nukleat virus encephalitis hutan |
| ROX | Kontrol Internal |
Parameter teknis
| Panyimpenan | ≤-18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | serum seger |
| Tt | ≤38 |
| CV | ≤5,0% |
| LoD | 500 eksemplar / ml |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315-R) dening Tiangen Biotech(Beijing) Co.,Ltd., ekstraksi kudu ditindakake miturut instruksi kanthi ketat. Volume sampel sing diekstrak sing disaranake yaiku 140μL lan volume elusi sing disaranake yaiku 60μL.
Reagen ekstraksi sing disaranake: Kit DNA/RNA Umum Makro & Uji Mikro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) lan Ekstraktor Asam Nukleat Otomatis Makro & Mikro (HWTSW6C-306) HWTS-3006B). extraction kudu conducted miturut instruksi strictly. Volume sampel sing diekstrak sing disaranake yaiku 200μL lan volume elusi sing disaranake yaiku 80μL.
