Wolung Jinis Virus Pernapasan
Jeneng produk
HWTS-RT184-Wolung Jinis Virus Pernapasan Kit Deteksi Asam Nukleat (PCR Fluoresensi)
Epidemiologi
Infeksi saluran pernapasan minangka jinis penyakit manungsa sing paling umum, sing bisa kedadeyan ing kabeh jinis kelamin, umur lan wilayah, lan minangka salah sawijining panyebab morbiditas lan kematian sing paling penting ing donya.[1]Patogen pernapasan sing umum sacara klinis kalebu virus influenza A (IFV A), virus influenza B (IFV B), virus respiratory syncytial, adenovirus, human metapneumovirus, rhinovirus, virus parainfluenza (I/II/III) lan mycoplasma pneumoniae, lan liya-liyane.[2,3]Gejala lan tandha klinis sing disebabake dening infeksi saluran pernapasan relatif padha, nanging infeksi sing disebabake dening patogen sing beda-beda nduweni metode perawatan, efek kuratif, lan perjalanan penyakit sing beda-beda.[4,5]Saiki, cara utama deteksi laboratorium patogen pernapasan kalebu: isolasi virus, deteksi antigen, lan deteksi asam nukleat. Kit iki ndeteksi lan ngenali asam nukleat virus tartamtu ing individu kanthi tandha lan gejala infeksi pernapasan, bebarengan karo asil klinis lan laboratorium liyane kanggo mbantu diagnosis infeksi virus pernapasan.
Parameter Teknis
| Panyimpenan | 2-8℃ |
| Umur simpan | 12 sasi |
| Jinis Spesimen | Swab orofaringeal; Swab nasofaring |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 Salinan/mL |
| Spesifisitas | Reaktivitas silang: Ora ana reaktivitas silang antarane kit lan virus Boca, Cytomegalovirus, virus Epstein-Barr, virus Herpes simplex, Virus varicella zoster, Virus gondongan, Enterovirus, virus campak, coronavirus manungsa, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, DNA genomik manungsa. Tes interferensi: Pilih musin (60mg/mL), getih manungsa (50%), benefrin (2mg/mL), hidroksimetazolin (2mg/mL) 2mg/mL), natrium klorida nganggo pengawet 5% (20mg/mL), beklometason (20mg/mL), deksametason (20mg/mL), fluniaseton (20μg/mL), triamsinolon (2mg/mL), budesonida (1mg/mL), mometason (2mg/mL), flutikason (2mg/mL), histamin hidroklorida (5mg/mL), benzokain (10%), mentol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirosin (20mg/mL), tobramisin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Asil nuduhake yen zat-zat sing ngganggu ing konsentrasi ing ndhuwur |
| Instrumen sing Ditrapake | Ditrapake kanggo reagen deteksi tipe I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Sistem PCR Wektu Nyata, Sistem PCR Wektu Nyata SLAN-96P (Hongshi Medical Technology Co., Ltd.), Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A, teknologi Hangzhou Bioer), Sikler Termal Kuantitatif Wektu Nyata MA-6000 (Suzhou Molarray Co., Ltd.), Sistem PCR Wektu Nyata BioRad CFX96, Sistem PCR Wektu Nyata BioRad CFX Opus 96. Ditrapake kanggo reagen deteksi tipe II: EudemonTM AIO800 (HWTS-EQ007) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Alur Kerja
Kit DNA/RNA Viral Tes Makro & Mikro (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Tes Makro & Mikro (HWTS-3006C, HWTS-3006B)), lan Kit DNA/RNA Viral Tes Makro & Mikro (HWTS-3017-8) (sing bisa digunakake karo EudemonTM AIO800 (HWTS-EQ007)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Volume sampel sing diekstrak yaiku 200μL lan volume elusi sing disaranake yaiku 150μL.
