Wolung Jinis Virus Pernafasan
jeneng produk
HWTS-RT184-Wolung Jinis Kit Deteksi Asam Nukleat Virus Pernafasan (Fluoresensi PCR)
Epidemiologi
Infeksi saluran pernapasan minangka jinis penyakit manungsa sing paling umum, sing bisa kedadeyan ing jender, umur lan wilayah apa wae, lan minangka salah sawijining panyebab morbiditas lan pati sing paling penting ing donya.[1]. Patogen pernapasan sing umum sacara klinis kalebu virus influenza A (IFV A), virus influenza B (IFV B), virus respiratory syncytial, adenovirus, metapneumovirus manungsa, rhinovirus, virus parainfluenza (I/II/III) lan mycoplasma pneumoniae, lsp.[2,3]. Gejala klinis lan pratandha sing disebabake infeksi saluran pernapasan relatif padha, nanging infeksi sing disebabake dening patogen sing beda nduweni cara perawatan, efek kuratif lan penyakit sing beda.[4,5]. Saiki, cara utama deteksi laboratorium patogen pernapasan kalebu: isolasi virus, deteksi antigen lan deteksi asam nukleat. Kit iki ndeteksi lan ngenali asam nukleat virus tartamtu ing individu sing duwe tandha lan gejala infeksi pernapasan, bebarengan karo asil klinis lan laboratorium liyane kanggo mbantu diagnosa infeksi virus pernapasan.
Parameter teknis
| Panyimpenan | 2-8 ℃ |
| urip beting | 12 wulan |
| Tipe Spesimen | swab oropharyngeal; Swab nasofaring |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 eksemplar/mL |
| Kekhususan | Reaktivitas silang: Ora ana reaktivitas silang antarane kit lan virus Boca, Cytomegalovirus, virus Epstein-Barr, virus Herpes simplex, Virus varicella zoster, virus Mumps, Enterovirus, virus campak, coronavirus manungsa, Coronavirus SARS, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Klebsigene Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Corynebacillus human, Lactobacillus DNA. Tes interferensi: Select musin (60mg/mL), getih manungsa (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride with 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), deksametason (20mg/mL) (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), flutikason (2mg/mL), histamin hidroklorida (5mg/mL), benzocaine (10%), mentol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpiromL (20mg/L), oseltamivir (60ng/mL), ribavirin (10mg/L), Asil nuduhake yen zat interfering ing konsentrasi ndhuwur |
| Instrumen sing ditrapake | Ditrapake kanggo reagen deteksi tipe I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Sistem PCR Real-Time, Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Sistem Deteksi PCR Real-Time (FQD-96A, teknologi Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Realtime CFXRTime CFX96), System Opus 96 Sistem PCR Real-Time. Ditrapake kanggo reagen deteksi jinis II: EudemonTM AIO800 (HWTS-EQ007) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Alur Kerja
Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Nukleat Otomatis Makro & Mikro (HWTS-3006C, HWTS-3006B)), lan Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-8 bisa digunakake)TM AIO800 (HWTS-EQ007)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Volume sampel sing diekstrak yaiku 200μL lan volume elusi sing disaranake yaiku 150μL.
