Chlamydia Trachomatis, Neisseria Gonorrhoeae lan Trichomonas vaginalis
jeneng produk
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae lan Trichomonas vaginalis Kit Deteksi Asam Nukleat (Fluoresensi PCR)
Epidemiologi
Chlamydia trachomatis (CT) minangka jinis mikroorganisme prokariotik sing parasit banget ing sel eukariotik.Chlamydia trachomatis dipérang dadi serotipe AK miturut metode serotipe.Infeksi saluran urogenital biasane disebabake dening serotipe DK varian biologis trachoma, lan wong lanang biasane diwujudake minangka uretritis, sing bisa diilangi tanpa perawatan, nanging umume dadi kronis, saya tambah sacara periodik, lan bisa digabung karo epididymitis, proctitis, lan liya-liyane.
Saluran
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Vaginitis trichomonal |
| VIC/HEX | Kontrol internal |
Parameter teknis
| Lumbung | -18 ℃ |
| urip beting | 12 sasi |
| Tipe Spesimen | swab serviks wanita,swab vagina wanita,Swab uretra lanang |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Salinan / mL |
| Kekhususan | Ora ana reaksi silang karo patogen infeksi STD liyane ing njaba kit deteksi kit, kayata Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus tipe 1, Herpes simplex virus tipe 2, Candida albicans, lsp. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Reagen ekstraksi sing disaranake: Pipet 1mL saka sampel sing bakal dites menyang 1,5mL tabung centrifuge bebas DNase/RNase, centrifuge ing 12000rpm suwene 3 menit, buang supernatan lan tetep endapan.Tambah 200µL normal saline menyang endapan kanggo resuspensi.Kit DNA/RNA Umum Makro & Mikro (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Makro & Mikro (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ekstraksi kudu ditindakake miturut pandhuan kanggo nggunakake.Volume sampel sing diekstrak yaiku 200µL, lan volume elusi sing disaranake yaiku 80µL.
Pilihan 2.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP302).Ekstraksi kudu ditindakake kanthi ketat miturut pandhuan kanggo nggunakake.Volume elusi sing disaranake yaiku 80µL.
