Gen Gene Gen KarbaMenem (KPC / NDM / OXA 48 / OXA 23 / Vim / Imp)
Jeneng Produk
HWTTS-OT0455 Gen karbenem (KPC / NDM / OXA 48 / OXA 23 / Vim / Imp) deteksi Kit (Fluorescence PCR)
Epidemiologi
Karbapenem antibiotik minangka antibiotik β-lactam atypical kanthi spektrum antibakteri sing paling gedhe lan kegiatan antibakteri sing paling kuat. Amarga stabilitas kanggo β-lactamase lan keracunan rendah, wis dadi salah sawijining obat antibakteri sing paling penting kanggo perawatan infeksi bakteri sing abot. CarbaPenerems pancen stabil banget kanggo plasmid - nengah-mediasi-spektrum lengkap-spektrum β-lactimases (ESBLS), kromosom, lan cephosor sing madhang plasmid (enzim ampc).
Saluran
| Pcr-mix 1 | Pcr-mix 2 | |
| Fames | Imp | Vim |
| Vic / Hex | Kontrol internal | Kontrol internal |
| CY5 | NDm | Kpc |
| Rok | Oxa48
| Oxa23 |
Paramèter teknis
| Panyimpen | ≤-18 ℃ |
| Beting urip | 12 wulan |
| Jinis spesimen | Sputum, Koloni Murni, Rectal Swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 103CFU / ml |
| Kekhususan | a) Kit kasebut ndeteksi referensi negatif perusahaan standar, lan asil kasebut cocog karo referensi sing cocog. b) Asil tes salib-reaktif nuduhake manawa kit iki ora ana reaksi salib karo patogen pernuk liyane, kayata pneumoniae, staphylococcus aureus, klebsiella osytoca, Influenzae Haemophilus, Acinetobcter Juniii, Acinetobacter Haemolyticus, Legionella Pneumophila, Escherichia CLOL, Peumoniae Pneumonia, Enterovirus, Enterocires, Enterocrus, EntoCiroprat, Enterocir, Gen, CTX, Sme, Shv, tem, lsp. c) Mucin, Minokinsi, Minokycline, Gentamicin, Clindamycin, Imipenem, Bahan Clafloxacin, lan Asil nuduhake manawa bahan campur tangan kasebut ora ana reaksi kanggo deteksi saka Karbenem resistensi genes kpc, ddm, oxa48, oxa23, vim, lan imp. |
| Instrumen sing ditrapake | Biosistem Applied 7500 Sistem PCR nyata-wektu Sistem PCR nyata-wektu SLAN-96P System PCR-Time-Time (Technology Technology HONGSHI CH, Ltd) Sistem PCR nyata-wektu Plancene 9600 Plus sistem deteksi PCR nyata-wektu (FQD-96A,HangzhouTeknologi Bioer) MA-6000 Masmal Cupter Thermal Real-Time-Time-Time-Time-Time (Suzhou Molarray Co, Ltd) Sistem PCR nyata-wektu Biorad CFX96 Sistem PCR nyata-wektu 96 |
Aliran kerja
Pilihan 1.
Reagent Extraction Disaranake: Umum Macro & Micro-Test DNA / RNA Kit (HWTS-3019-50, hwts-3019-32, hwts-3019-48, HWTS-3019-96) (sing bisa digunakake karo Macro-Test Eksport Asam National Acter (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Tes Micro-Test Med-Tech Co, Ltd .. Tambah 200-Uji Biasa kanggo The Thallus mendak. Langkah sabanjure kudu ngetutake pandhuan kanggo ekstraksi, lan volume zat sing disaranake yaiku100μl.
Pilihan 2.
Reagent Extraction Disaranake: Aktraksi Asam Nukleat utawa Purifikasi (YDP302) dening Tiangen Biotech (Beijing) Co, Ltd instruksi kanggo nggunakake 2 instruksi kanggo digunakake (Tambah 200) , lan goyang nganti thallus wis rampung). Gunakake banyu gratis rnase / lik kanggo volir, lan volume empan sing eCommended yaiku 100μl.
Pilihan 3.
Reagent Extraction: Nilai Tes Macro & Micro-Micro Micro-Micro Micro. Sampel sputum kudu dikumbah kanthi nambah 1ml uyah normal kanggo thallus endapan sing disedhiyakake ing ndhuwur, centrifuged ing 13000r / min sajrone 5 menit, lan supernatant 10-20orl). Kanggo koloni murni lan rectal Swab, tambahake 50 anlong sampel ngeculake reagen langsung menyang thallus mendakan sing disedhiyakake ing ndhuwur, lan langkah sabanjure kudu dijupuk miturut instruksi sing dienggo.
