Gen Resistensi Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
jeneng produk
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kit Deteksi (Fluoresensi PCR)
Epidemiologi
Antibiotik Carbapenem minangka antibiotik β-laktam atipikal kanthi spektrum antibakteri sing paling jembar lan aktivitas antibakteri sing paling kuat.Amarga stabilitas kanggo β-laktamase lan keracunan kurang, wis dadi salah sawijining obat antibakteri sing paling penting kanggo perawatan infeksi bakteri sing abot.Carbapenems stabil banget kanggo plasmid-mediated extended-spectrum β-laktamase (ESBLs), kromosom, lan plasmid-mediated cephalosporinase (enzim AmpC).
Saluran
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Kontrol internal | Kontrol internal |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Parameter teknis
| Lumbung | ≤-18 ℃ |
| urip beting | 12 sasi |
| Tipe Spesimen | Sputum, koloni murni, swab rektum |
| Ct | ≤36 |
| CV | ≤5,0% |
| LoD | 103CFU/mL |
| Kekhususan | a) Kit ndeteksi referensi negatif perusahaan standar, lan asil kasebut cocog karo syarat referensi sing cocog. b) Asil tes reaktivitas silang nuduhake yen kit iki ora duwe reaksi silang karo patogen pernapasan liyane, kayata Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenza. junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, utawa conto sing ngemot gen tahan obat liyane CTX, mecA, SME, SHV, TEM. c) Anti-interferensi: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin dipilih kanggo tes interferensi, lan asil nuduhake yen reaksi interfering kasebut ing ndhuwur ora duwe interfering. kanggo deteksi gen resistensi carbapenem KPC, NDM, OXA48, OXA23, VIM, lan IMP. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time QuantStudio®5 Sistem PCR Wektu Nyata Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.) Sistem PCR LightCycler®480 Real-Time Sistem Deteksi PCR Wektu Nyata LineGene 9600 Plus (FQD-96A,Hangzhouteknologi bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Reagen ekstraksi sing disaranake: Kit DNA/RNA Umum Makro & Uji Mikro (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (sing bisa digunakake karo Ekstraktor Asam Nukleat Otomatis Macro & Micro-Test (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Tambah 200μL saline normal menyang endapan thallus.Langkah-langkah sabanjure kudu ngetutake instruksi kanggo ekstraksi, lan volume elusi sing disaranake yaiku100μL.
Pilihan 2.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Reagen Pemurnian (YDP302) dening Tiangen Biotech (Beijing) Co., Ltd. Ekstraksi kudu diwiwiti kanthi ketat karo langkah 2 instruksi panggunaan (nambahake 200μL buffer GA menyang endapan thallus , lan goyangake nganti thallus rampung digantung).Gunakake banyu bebas RNase/DNase kanggo elusi, lan volume elusi sing disaranake yaiku 100μL.
Pilihan 3.
Reagen ekstraksi sing disaranake: Reagen Pelepasan Sampel Makro & Tes Mikro.Sampel sputum kudu dicuci kanthi nambahake 1mL saline normal menyang endapan thallus sing wis kasebut ing ndhuwur, disentrifugasi ing 13000r / min suwene 5 menit, lan supernatan dibuwang (simpen 10-20μL supernatant).Kanggo koloni murni lan swab rektum, nambah 50μL saka sampel release reagen langsung menyang ndhuwur-kasebut dianggep thallus precipitate, lan langkah-langkah sakteruse kudu dijupuk miturut instruction kanggo nggunakake.
