4 Jenis Virus Respiratorius
jeneng produk
HWTS-RT099- 4 Jinis Kit Deteksi Asam Nukleat Virus Pernafasan (Fluoresensi PCR)-内参是NED-ABI 7500 Sistem PCR Wektu Nyata/ABI 7500 Sistem PCR Wektu Nyata Cepet/QuantStudio®5 Sistem PCR Real-Time
HWTS-RT158-4 Jenis Virus Pernafasan Kit Deteksi Asam Nukleat (Fluoresensi PCR)-内参Quasar 705
Epidemiologi
Penyakit Virus Corona 2019, diarani "COVID-19", nuduhake pneumonia sing disebabake dening2019-nCoVinfeksi.2019-nCoVyaiku coronavirus sing kalebu genus β. COVID-19 minangka penyakit infèksi pernapasan akut, lan populasi umume rentan. Saiki, sumber infeksi utamane yaiku pasien sing kena infeksi2019-nCoV, lan wong sing kena infeksi asimtomatik uga bisa dadi sumber infeksi. Adhedhasar penyelidikan epidemiologis saiki, periode inkubasi yaiku 1-14 dina, biasane 3-7 dina. Demam, batuk garing lan lemes minangka manifestasi utama. Sawetara pasien duwe gejalas kayatanasal congestion, irung runny, perih tenggorokan, myalgia lan diare, lsp.
Saluran
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kontrol Internal |
Parameter teknis
| Panyimpenan | -18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Salinan/mLVirus Influenza A/Virus Influenza B/Virus Respiratory Syncytial: 500 Salinan/mL |
| Kekhususan | a) Asil reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit lan coronavirus manungsa SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virus parainfluenza tipe 1, 2, 3, rhinovirus A, B, C, virus mochlamyne enteritis. B, C, D, virus paru-paru manungsa, virus epstein-barr, virus campak, virus sitomegalo manungsa, rotavirus, norovirus, virus parotitis, virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyococcus pneumoniae, streptococcus pyococcus pneumoniae kumelun aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci lan cryptococcus bayi lan asam nukleat genomik manungsa. b) Kemampuan anti-interferensi: pilih musin (60mg/mL), 10% (v/v) getih lan phenylephrine (2mg/mL), oxymetazoline (2mg/mL), natrium klorida (kalebu pengawet) (20 mg/mL), beclomethasone (20mg/mL), deksametason (20mg/mL), deksametason (20mg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL) (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) lan tes interference (10μg/mL) lan for. asil nuduhake yen zat interfering karo konsentrasi kasebut ing ndhuwur ora reaksi interferensi kanggo asil test saka patogen. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) lan Ekstraktor Asam Nukleat Nukleat Otomatis Macro & Micro-Test (HWTS-3006) diprodhuksi dening Jiangsu Macro & Micro-Test Med-Teched Co., Ltd. volume punika 80μL.
Pilihan 2.
Kit Mini RNA Viral QIAamp (52904) diprodhuksi dening QIAGEN utawa Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315-R) diprodhuksi dening Tiangen Biotech (Beijing) Co., Ltd. Volume sampel sing diekstrak yaiku 140μL, lan volume elusi sing disaranake yaiku 60μL.
