4 Jenis Virus Respiratorius
jeneng produk
HWTS-RT099- 4 Jenis Virus Pernafasan Kit Deteksi Asam Nukleat (Fluoresensi PCR)
Epidemiologi
Penyakit Virus Corona 2019, diarani "COVID-19", nuduhake pneumonia sing disebabake dening2019-nCoVinfeksi.2019-nCoVyaiku coronavirus sing kalebu genus β.COVID-19 minangka penyakit infèksi pernapasan akut, lan populasi umume rentan.Saiki, sumber infeksi utamane yaiku pasien sing kena infeksi2019-nCoV, lan wong sing kena infeksi asimtomatik uga bisa dadi sumber infeksi.Adhedhasar penyelidikan epidemiologis saiki, periode inkubasi yaiku 1-14 dina, biasane 3-7 dina.Demam, batuk garing lan lemes minangka manifestasi utama.Sawetara pasien duwe gejalas kayatanasal congestion, irung runny, perih tenggorokan, myalgia lan diare, lsp.
Saluran
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kontrol internal |
Parameter teknis
| Lumbung | -18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Salinan/mLVirus Influenza A/Virus Influenza B/Virus Respiratory Syncytial: 500 Salinan/mL |
| Kekhususan | a) Asil reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit lan coronavirus manungsa SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virus parainfluenza tipe 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, asap aspergillus, candida albicans, candida glabrata, pneumocystis cryptomic jirocicoccus lan asam gen. b) Kemampuan anti-interferensi: pilih musin (60mg/mL), 10% (v/v) getih lan phenylephrine (2mg/mL), oxymetazoline (2mg/mL), natrium klorida (kalebu pengawet) (20 mg/mL). ), beclomethasone (20mg/mL), deksametason (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL). ), histamin hidroklorida (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/ mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) lan tobramycin (0,6mg/mL). mL) kanggo tes interferensi, lan asil nuduhake yen zat sing ngganggu konsentrasi kasebut ing ndhuwur ora duwe reaksi interferensi marang asil tes patogen. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Sistem PCR Wektu Nyata |
Alur Kerja
Pilihan 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) lan Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) diprodhuksi dening Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. Volume sampel sing diekstrak yaiku 200μL, lan volume elusi sing disaranake yaiku 80μL.
Pilihan 2.
Kit Mini RNA Viral QIAamp (52904) diprodhuksi dening QIAGEN utawa Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315-R) diprodhuksi dening Tiangen Biotech (Beijing) Co., Ltd. Volume sampel sing diekstrak yaiku 140μL, lan volume elusi sing disaranake yaiku 60μL.
