29 Jinis Asam Nukleat Gabungan Patogen Pernafasan
jeneng produk
HWTS-RT160 -29 Jinis Kit Deteksi Asam Nukleat Gabungan Patogen Pernafasan
Epidemiologi
Infeksi saluran pernapasan minangka penyakit sing paling umum ing manungsa, sing bisa kedadeyan ing kabeh jinis, umur lan wilayah. Iki minangka salah sawijining panyebab utama morbiditas lan mortalitas ing populasi ing saindenging jagad [1]. Patogen pernapasan sing umum kalebu coronavirus novel, virus Influenza A, virus Influenza B, Virus syncytial Respiratory, Adenovirus, metapneumovirus manungsa, rhinovirus, virus Parainfluenza tipe I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, lan Streptococcus pneumoniae, lsp[2,3]. Gejala lan pratandha sing disebabake dening infeksi pernapasan relatif padha, nanging cara perawatan, khasiat lan cara infeksi sing disebabake dening patogen sing beda beda [4,5]. Saiki, cara utama sing digunakake ing laboratorium kanggo ndeteksi patogen pernapasan sing kasebut ing ndhuwur kalebu: isolasi virus, deteksi antigen lan deteksi asam nukleat, lan liya-liyane. Asil negatif ora ngilangi infeksi virus pernapasan lan ora bisa digunakake minangka basis tunggal kanggo diagnosis, perawatan, utawa keputusan manajemen liyane. Asil positif ora bisa ngilangi infeksi bakteri utawa infeksi campuran dening virus liya sing ana ing njaba indikator tes. Operator eksperimen kudu nampa latihan profesional babagan amplifikasi gen utawa deteksi biologi molekuler, lan nduweni kualifikasi operasi eksperimental sing relevan. Laboratorium kudu duwe fasilitas pencegahan biosafety lan prosedur perlindungan sing cukup.
Parameter teknis
| Panyimpenan | -18 ℃ |
| urip beting | 9 wulan |
| Tipe Spesimen | Sapuan tenggorokan |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 eksemplar / μL |
| Kekhususan | Asil tes reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit iki lan Cytomegalovirus, virus Herpes simplex tipe 1, virus Varicella-zoster, virus Epstein-Barr, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilar at Mycobacterium strains. tuberkulosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderystrium cepacratia, No. marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, lan asam nukleat genomik manungsa. |
| Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-Time, Applied Biosystems 7500 Sistem PCR Wektu Nyata Cepet, QuantStudio®5 Sistem PCR Real-Time, Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Sistem PCR Real-Time, LineGene 9600 Plus Sistem Deteksi PCR Wektu Nyata (FQD-96A, teknologi Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Sistem PCR BioRad CFX96 Real-Time, BioRad CFX Opus 96 Sistem PCR Real-Time. |
Alur Kerja
Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (sing bisa digunakake karo Ekstraktor Asam Nukleat Nukleat Otomatis Makro & Mikro (HWTS-3006C, HWTS-3006B)), lan Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017-8 bisa digunakake)TM AIO800 (HWTS-EQ007)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Volume sampel sing diekstrak yaiku 200μL lan volume elusi sing disaranake yaiku 150μL.
