12 Jinis Patogen Pernafasan
jeneng produk
HWTS-RT071A 12 Jinis Kit Deteksi Asam Nukleat Patogen Pernafasan (PCR Fluoresensi)
Saluran
Saluran | hu12Reaksi Buffer A | hu12Reaksi Buffer B | hu12Reaksi Buffer C | hu12Reaksi Buffer D |
FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
VIC/HEX | Kontrol internal | Kontrol internal | HPIV Ⅱ | Kontrol internal |
CY5 | IFV A | MP | HPIV Ⅲ | / |
ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Parameter teknis
Lumbung | ≤-18 ℃ |
urip beting | 12 sasi |
Tipe Spesimen | Oropharyngeal swab |
Ct | ≤38 |
CV | ≤5,0% |
LoD | SARS-CoV-2: 300 Salinan/mLvirus influenza B: 500 Salinan/mLvirus influenza A: 500 Salinan/mL Adenovirus: 500 Copy/mL mycoplasma pneumoniae: 500 Salinan / mL respiratory syncytial virus: 500 Copy/mL, virus parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): 500 Salinan/mL Rhinovirus: 500 Copy/mL metapneumovirus manungsa: 500 Salinan / mL |
Kekhususan | Panaliten reaktivitas silang nuduhake yen ora ana reaktivitas silang antarane kit iki lan enterovirus A, B, C, D, virus epstein-barr, virus campak, sitomegalovirus manungsa, rotavirus, norovirus, virus mumps, virus varicella-herpes zoster, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans lan asam nukleat genomik manungsa. |
Instrumen sing ditrapake | Applied Biosystems 7500 Sistem PCR Real-TimeApplied Biosystems 7500 Fast Real-Time PCR SystemsQuantStudio®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sistem PCR Real-Time, LineGene 9600 Plus Sistem Deteksi PCR Wektu Nyata (FQD-96A, teknologi Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time |
Alur Kerja
Pilihan 1.
Reagen ekstraksi sing disaranake: Reagen Pelepasan Sampel Macro & Micro-Test (HWTS-3005-8) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ekstraksi kudu ditindakake kanthi ketat miturut pandhuane.
Pilihan 2.
Reagen ekstraksi sing disaranake: Kit DNA/RNA Umum Makro & Uji Mikro (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) lan Ekstraktor Asam Nukleat Otomatis Makro & Uji Mikro ( HWTS-3006C, HWTS-3006B) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ekstraksi kudu ditindakake miturut instruksi kanthi ketat.Volume elusi sing disaranake yaiku 80μL.
Pilihan 3.
Reagen ekstraksi sing disaranake: Ekstraksi Asam Nukleat utawa Kit Pemurnian (YDP315) dening Tiangen Biotech(Beijing) Co., Ltd., ekstraksi kudu ditindakake miturut instruksi kanthi ketat.Volume elusi sing disaranake yaiku 100μL.