11 Jinis Patogen Pernafasan

11 Jinis Gambar Unggulan Patogen Pernafasan

Katrangan singkat:

Kit iki digunakake kanggo deteksi kualitatif in vitro saka patogen ambegan klinis umum ing sputum manungsa, kalebu Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonassis maltophilia (B. parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Leg). Asil kasebut bisa digunakake minangka pitulung kanggo diagnosa pasien sing dirawat ing rumah sakit utawa lara kritis sing dicurigai kena infeksi bakteri ing saluran pernapasan.Kit iki digunakake kanggo deteksi kualitatif in vitro saka patogen ambegan klinis umum ing sputum manungsa, kalebu Haemophilus influenzae (HI), Streptococcus pneumoniae (SP), Acinetobacter baumannii (ABA), Pseudomonas aeruginosa (PA), Klebsiella Pneumoniae (KPN), Stenotrophomonassis maltophilia (B. parapertusss (Bpp), Mycoplasma pneumoniae (MP), Chlamydia pneumoniae (Cpn), Legionella pneumophila (Leg). Asil kasebut bisa digunakake minangka pitulung kanggo diagnosa pasien sing dirawat ing rumah sakit utawa lara kritis sing dicurigai kena infeksi bakteri ing saluran pernapasan.

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HWTS-RT162A 11 Jinis Kit Deteksi Asam Nukleat Patogen Pernafasan(Fluoresensi PCR)

Saluran

靶标				Saluran
核酸反应液A	核酸反应液B	核酸反应液C	核酸反应液D	Saluran
HI	Smet	MP	sikil	FAM
SP	PA	Bp	/	CY5
KPN	ABA	Bpp	Cpn	ROX
内参	内参	内参	内参	VIC/HEX

Epidemiologi

Infeksi saluran pernapasan minangka kelas penyakit penting sing nyebabake ancaman serius kanggo kesehatan manungsa, lan panliten nuduhake manawa umume infeksi saluran pernapasan disebabake dening patogen bakteri lan utawa virus sing nginfèksi host, sing ndadékaké tambah keruwetan penyakit lan malah mati, saéngga njlentrehake patogen kasebut ngidini perawatan sing ditargetake lan ningkatake tingkat kaslametan pasien.^[1,2].

Parameter teknis

Panyimpenan	≤-18 ℃
urip beting	12 wulan
Tipe Spesimen	Sputum
Ct	HI, SP, KPN, PA, ABA, Smet: Ct≤33BP, Bpp, MP, Cpn, Sikil: Ct≤38
CV	<5,0%
LoD	Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasaeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia lan Legionellapneumophila: 1000 CFU/mL； Bordetella pertussis lan Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae lan Chlamydia pneumoniae: 200 eksemplar/mL.
Kekhususan	Asil tes reaktivitas silang nuduhake yen ora ana reaksi silang antarane kit iki lan Cytomegalovirus, virus Herpes simplex tipe 1, virus Varicella-zoster, virus Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilalis saka galur. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia, No. Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii lan inti genomik asam manungsa.
Instrumen sing ditrapake	Applied Biosystems 7500 Sistem PCR Real-Time Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®5 Sistem PCR Real-Time Sistem PCR Real-Time SLAN-96P LightCycler^®480 Sistem PCR Real-Time LineGene 9600 Plus Sistem Deteksi PCR Real-Time MA-6000 Real-Time Kuantitatif Thermal Cycler Sistem PCR BioRad CFX96 Real-Time BioRad CFX Opus 96 Sistem PCR Real-Time

Alur Kerja

Kit DNA/RNA Umum Makro & Mikro (HWTS-3019) (sing bisa digunakake karo Ekstraktor Asam Nukleat Nukleat Otomatis Makro & Mikro (HWTS-3006C, HWTS-3006B)) dening Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Tambah 200µL sakafisiologissaline menyang precipitate sing diproses, lan langkah-langkah sabanjure kudu ditindakake miturut pandhuane. Volume elusi sing disaranake yaiku 100 µL.

Sadurunge: Manungsa PML-RARA Fusion Gene Mutation

Sabanjure: Polimorfisme Genetik ALDH

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